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33342 pi double stain kit  (Beijing Solarbio Science)


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    Beijing Solarbio Science 33342 pi double stain kit
    33342 Pi Double Stain Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 308 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/33342+pi+double+staining+kit/pmc12805112-213-8-12?v=Beijing+Solarbio+Science
    Average 96 stars, based on 308 article reviews
    33342 pi double stain kit - by Bioz Stars, 2026-07
    96/100 stars

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    Pue alleviates cardiomyocyte pyroptosis via the NRF2/HO-1 signaling pathway. (A) <t>Hoechst</t> <t>33342/PI</t> staining detected the apoptotic state of H9C2 cells (magnification, ×400) (n=3). (B) Quantitative analysis of PI-positive cells. (C) Western blotting of nuclear NRF2 expression in each group (n=3). (D) Western blotting images of key pyroptotic proteins and HO-1 in each treatment group (n=3). (E) Semi-quantification of the expression of pyroptotic proteins and HO-1 in each group of cells. (F) Semi-quantification of NRF2 protein expression. ****P<0.0001 vs. OE-NC group; ### P<0.001 and #### P<0.0001 vs. H 2 O 2 + OE-NC group. Pue, puerarin; OE, overexpression; NC, negative control; NRF2, nuclear factor E2-related factor 2; ASC, apoptosis-associated speck-like protein; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D; HO-1, heme oxygenase-1.
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    Pue alleviates cardiomyocyte pyroptosis via the NRF2/HO-1 signaling pathway. (A) <t>Hoechst</t> <t>33342/PI</t> staining detected the apoptotic state of H9C2 cells (magnification, ×400) (n=3). (B) Quantitative analysis of PI-positive cells. (C) Western blotting of nuclear NRF2 expression in each group (n=3). (D) Western blotting images of key pyroptotic proteins and HO-1 in each treatment group (n=3). (E) Semi-quantification of the expression of pyroptotic proteins and HO-1 in each group of cells. (F) Semi-quantification of NRF2 protein expression. ****P<0.0001 vs. OE-NC group; ### P<0.001 and #### P<0.0001 vs. H 2 O 2 + OE-NC group. Pue, puerarin; OE, overexpression; NC, negative control; NRF2, nuclear factor E2-related factor 2; ASC, apoptosis-associated speck-like protein; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D; HO-1, heme oxygenase-1.
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    Average 96 stars, based on 1 article reviews
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    Image Search Results


    Pue alleviates cardiomyocyte pyroptosis via the NRF2/HO-1 signaling pathway. (A) Hoechst 33342/PI staining detected the apoptotic state of H9C2 cells (magnification, ×400) (n=3). (B) Quantitative analysis of PI-positive cells. (C) Western blotting of nuclear NRF2 expression in each group (n=3). (D) Western blotting images of key pyroptotic proteins and HO-1 in each treatment group (n=3). (E) Semi-quantification of the expression of pyroptotic proteins and HO-1 in each group of cells. (F) Semi-quantification of NRF2 protein expression. ****P<0.0001 vs. OE-NC group; ### P<0.001 and #### P<0.0001 vs. H 2 O 2 + OE-NC group. Pue, puerarin; OE, overexpression; NC, negative control; NRF2, nuclear factor E2-related factor 2; ASC, apoptosis-associated speck-like protein; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D; HO-1, heme oxygenase-1.

    Journal: Molecular Medicine Reports

    Article Title: Puerarin attenuates myocardial ischemia-reperfusion injury by inhibiting myocardium pyroptosis via the NRF2/HO-1 signaling pathway

    doi: 10.3892/mmr.2026.13824

    Figure Lengend Snippet: Pue alleviates cardiomyocyte pyroptosis via the NRF2/HO-1 signaling pathway. (A) Hoechst 33342/PI staining detected the apoptotic state of H9C2 cells (magnification, ×400) (n=3). (B) Quantitative analysis of PI-positive cells. (C) Western blotting of nuclear NRF2 expression in each group (n=3). (D) Western blotting images of key pyroptotic proteins and HO-1 in each treatment group (n=3). (E) Semi-quantification of the expression of pyroptotic proteins and HO-1 in each group of cells. (F) Semi-quantification of NRF2 protein expression. ****P<0.0001 vs. OE-NC group; ### P<0.001 and #### P<0.0001 vs. H 2 O 2 + OE-NC group. Pue, puerarin; OE, overexpression; NC, negative control; NRF2, nuclear factor E2-related factor 2; ASC, apoptosis-associated speck-like protein; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D; HO-1, heme oxygenase-1.

    Article Snippet: The Hoechst 33342/PI double fluorescence staining kit (cat. no. CA1120; Beijing Solarbio Science & Technology Co., Ltd.) was used to detect pyroptosis in H9C2 cardiomyocytes from each group.

    Techniques: Staining, Western Blot, Expressing, Over Expression, Negative Control, Binding Assay